--- This page is part of [Coy Laboratory Products](https://coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://coylab.com/llms.txt) or [Full Content Archive](https://coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Sulfate-Reducing Bacteria-Dominated Biofilms That Precipitate ZnS in a Subsurface Circumneutral-pH Mine Drainage System > Abstract The microbial diversity of ZnS-forming biofilms in 8C, circumneutral-pH groundwater in tunnels within the abandoned Piquette Zn, Pb mine (Tennyson,... **URL:** https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/ **Type:** Citation **Modified:** 2025-09-26 --- #### Abstract The microbial diversity of ZnS-forming biofilms in 8C, circumneutral-pH groundwater in tunnels within the abandoned Piquette Zn, Pb mine (Tennyson, Wisconsin, USA) has been investigated by molecular methods, fluorescence in situ hybridization (FISH), and cultivation techniques. These biofilms are growing on old mine timbers that generate locally anaerobic zones within the mine drainage system. Sulfate-reducing bacteria (SRB) exclusively of the family Desulfobacteriaceae comprise a significant fraction of the active microbiota. Desulfosporosinus strains were isolated, but could not be detected by molecular methods. Other important microbial clusters belonged to the b-, c-, and e-Proteobacteria, the Cytophaga/Flexibacter/Bacteroides-group (CFB), Planctomycetales, Spirochaetales, Clostridia, and green nonsulfur bacteria. Our investigations indicated a growth dependence of SRB on fermentative, cellulolytic, and organic acid-producing Clostridia. A few clones related to sulfuroxidizing bacteria were detected, suggesting a sulfur cycle related to redox gradients within the biofilm. Sulfur oxidation prevents sulfide accumulation that would lead to precipitation of other sulfide phases. FISH analyses indicated that Desulfobacteriaceae populations were not early colonizers in freshly grown and ZnS-poor biofilms, whereas they were abundant in older, naturally established, and ZnS-rich biofilms. Gram-negative SRB have been detected in situ over a period of 6 months, supporting the important role of these organisms in selective ZnS precipitation in Tennyson mine. Results demonstrate the complex nature of biofilms responsible for in situ bioremediation of toxic metals in a subsurface mine drainage system. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. [AI Content Index](https://coylab.com/llms.txt) | [Full Site Content](https://coylab.com/llms-full.txt) | [Entity Card](https://coylab.com/wp-json/bc-geodesic/v1/entity-card) ```json {"@context":"https://schema.org","@graph":[{"@type":"Organization","@id":"https://coylab.com/#organization","name":"Coy Laboratory Products","url":"https://coylab.com","description":"From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.","disambiguatingDescription":"Laboratory equipment manufacturing company specializing in vinyl glove boxes","logo":{"@type":"ImageObject","url":"https://coylab.com/wp-content/uploads/2025/06/coylab-header_logo-svg.svg"},"address":{"@type":"PostalAddress","streetAddress":"14500 Coy Drive","addressLocality":"Grass Lake","addressRegion":"MI","postalCode":"49240"},"telephone":"734.475.2200","email":"sales@coylab.com","foundingDate":"1969","areaServed":"Global","sameAs":["https://www.linkedin.com/company/107172311","https://maps.app.goo.gl/o2txKDMScV1pV7w59","https://www.pharmaceutical-tech.com/suppliers/coy-laboratory","https://craft.co/coy-laboratory-products-inc"],"founder":[{"@type":"Person","name":"Richard Coy"},{"@type":"Person","name":"Brian Coy","sameAs":"https://www.linkedin.com/in/brian-coy-5722515/"},{"@type":"Person","name":"Ashley Coy","sameAs":"https://www.linkedin.com/in/ashley-coy-6b22705/"}],"knowsAbout":[{"@type":"Thing","name":"laboratory equipment","sameAs":"https://www.wikidata.org/wiki/Q834028"},"vinyl anaerobic chambers","vinyl hypoxic chambers","laboratory glove boxes","laboratory chamber sensors",{"@type":"Thing","name":"life sciences","sameAs":"https://www.wikidata.org/wiki/Q864928"},{"@type":"Thing","name":"microbiology","sameAs":"https://www.wikidata.org/wiki/Q7193"},{"@type":"Thing","name":"medical research","sameAs":"https://www.wikidata.org/wiki/Q2752427"}]},{"@type":"WebSite","@id":"https://coylab.com/#website","name":"Coy Laboratory Products","url":"https://coylab.com","publisher":{"@id":"https://coylab.com/#organization"},"potentialAction":{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://coylab.com/?s={search_term_string}"},"query-input":"required name=search_term_string"},"hasPart":[{"@type":"DigitalDocument","name":"LLM Content Index","url":"https://coylab.com/llms.txt","encodingFormat":"text/markdown","description":"AI-readable index of site content, entity data, and subject matter expertise"},{"@type":"DigitalDocument","name":"LLM Full Content","url":"https://coylab.com/llms-full.txt","encodingFormat":"text/markdown","description":"Complete AI-readable site content"}]},{"@type":"ItemList","@id":"https://coylab.com/#site-navigation","name":"Site Navigation","itemListElement":[{"@type":"SiteNavigationElement","position":1,"name":"Products","url":"#"},{"@type":"SiteNavigationElement","position":2,"name":"Applications","url":"#"},{"@type":"SiteNavigationElement","position":3,"name":"Custom","url":"#"},{"@type":"SiteNavigationElement","position":4,"name":"Sales","url":"#"},{"@type":"SiteNavigationElement","position":5,"name":"Resources","url":"#"},{"@type":"SiteNavigationElement","position":6,"name":"Contact Us","url":"#"}]},{"@type":"BreadcrumbList","@id":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://coylab.com"},{"@type":"ListItem","position":2,"name":"Citations","item":"https://coylab.com/research-citations/"},{"@type":"ListItem","position":3,"name":"Anaerobic Chambers","item":"https://coylab.com/citation-category/anaerobic-chambers/"},{"@type":"ListItem","position":4,"name":"Sulfate-Reducing Bacteria-Dominated Biofilms That Precipitate ZnS in a Subsurface Circumneutral-pH Mine Drainage System"}]},{"@type":"WebPage","@id":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/#webpage","url":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/","name":"Sulfate-Reducing Bacteria-Dominated Biofilms That Precipitate ZnS in a Subsurface Circumneutral-pH Mine Drainage System | Coy Laboratory Products","isPartOf":{"@id":"https://coylab.com/#website"},"breadcrumb":{"@id":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/#breadcrumb"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","about":[{"@type":"Thing","name":"Anaerobic Chambers"}]},{"@type":"ScholarlyArticle","@id":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/#scholarlyarticle","headline":"Sulfate-Reducing Bacteria-Dominated Biofilms That Precipitate ZnS in a Subsurface Circumneutral-pH Mine Drainage System","url":"https://coylab.com/research-citation/sulfate-reducing-bacteria-dominated-biofilms-that-precipitate-zns-in-a-subsurface-circumneutral-ph-mine-drainage-system/","datePublished":"2025-09-16T16:11:05+00:00","dateModified":"2025-09-26T16:16:44+00:00","isPartOf":{"@id":"https://coylab.com/#website"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","author":{"@id":"https://coylab.com/author/buildcreate/#person"},"publisher":{"@id":"https://coylab.com/#organization"}},{"@type":"Person","@id":"https://coylab.com/author/buildcreate/#person","name":"buildcreate","url":"https://coylab.com/author/buildcreate/"}]} ```