--- This page is part of [Coy Laboratory Products](https://coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://coylab.com/llms.txt) or [Full Content Archive](https://coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # A novel human enteroid-anaerobe co-culture system to study microbial-host interaction under physiological hypoxia > Abstract Mechanistic investigations of host-microbe interactions in the human gut are limited by current coculture model systems. The intestinal epithelium... **URL:** https://coylab.com/research-citation/a-novel-human-enteroid-anaerobe-co-culture-system-to-study-microbial-host-interaction-under-physiological-hypoxia-2/ **Type:** Citation **Modified:** 2025-09-26 --- #### Abstract Mechanistic investigations of host-microbe interactions in the human gut are limited by current coculture model systems. The intestinal epithelium requires oxygen for viability, while gut bacteria are facultative or obligate anaerobes. The ability to model host-commensal interactions under dynamic oxygen conditions is critical to understanding host-pathogen interactions in the human gut. Here, we demonstrate a simple, cost-effective method for co-culturing obligate anaerobic bacteria with human intestinal enteroid monolayers under variable oxygen conditions. The enteroid-Anaerobe Co-Culture (EACC) system is able to recapitulate the steep oxygen gradient seen in vivo and induce expression of hypoxia-associated phenotypes such as increased barrier integrity and expression of antimicrobial peptide genes. Using clinical strains of the commensal anaerobes Bacteroides thetaiotaomicron and Blautia sp. on established patient-derived intestinal enteroid cell lines under physiological hypoxia, the EACC system can sustain host-anaerobe interactions for at least 24 hours. Following co-culture with anarobic bacteria, we demonstrate patient-specific differences in epithelial response, reinforcing the potential to develop a personalized medicine approach to bacteriotherapy and host-microbe interaction investigations. Our innovative EACC system provides a robust model for investigating host-microbe interactions in complex, patient-derived intestinal tissues, that facilitates study of mechanisms underlying the role of the microbiome in health and disease. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. 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